Application of Magnetizable Cellulose Particles in Radioimmunoassay for In-Vitro Determination of Follicle Stimulating Hormone in Human Serum

The measurement of Follicle Stimulating Hormone (FSH) in human serum is essential for investigating fertility and especially disorders of the hypothalamic pituitary gonadal axis. Therefore, the present study aimed to prepare radioimmunoassay (RIA) system using solid phase magnetic particles for the invitro quantitative measurement of FSH in human serum. The preparation of polyclonal antibody (anti-FSH), I-FSH tracer and FSH standards was carried out. The activation of magnetic particles using 1, 1-carbonyl diimidazole (CDI) and coupling of activated particles with purified anti-FSH were carried out. Optimization and validation of the assay were undertaken. The reproducibility as measured by the intraand inter-assay variations is acceptable. The recovery and dilution tests indicated accurate calibration and appropriate matrix. The present technique is in a good agreement well with the currently used commercial kit (Izotop, IRMA). The magnetic particles of the present system for estimation of FSH retain their characteristics during storage for 12 months at 4 °C. It can be concluded that this technique proved to be sensitive, specific, precise and accurate for routine laboratory use.